Fig. 1. Fatty acid composition and cell viability of peroxisome-deficient CHO cells incubated with various VLCFAs.CHO-zp102 (peroxisome-deficient) cells were incubated in serum-free medium with the indicated concentrations of VLCFAs. Fatty acid levels were analyzed at 24 h by gas chromatography (A). Cell viability was assessed at 72 h by trypan blue dye exclusion test (A). Cytotoxicities of different VLCFAs at different concentrations were plotted against decreased levels of C18:1 in cellular lipids (B). Values represent the mean ± S.D. of three independent experiments. Statistical significance was determined using one-way analysis of variance (ANOVA) followed by the Tukey’s post-hoc test or Student’s t-test. *p < 0.05 indicates a significant difference between indicated groups.Protective and non-protective effects of Lorenzo’s oil componentsWe investigated whether the components of Lorenzo’s oil, C18:1 and C22:1, could protect against VLCFA-induced apoptosis. Treatment with C24:0, C24:1, C26:0, or C26:1 significantly decreased cell viability. Supplementation with C18:1 dose-dependently rescued cell viability under all conditions (Fig. 2A). In contrast, C22:1 showed no protective effect and instead enhanced VLCFA cytotoxicity (Fig. 2B). Furthermore, co-supplementation with C22:1 reduced the protective effect of C18:1 (Fig. 2A). These findings suggest that C22:1, a key component of Lorenzo’s oil, may be harmful to peroxisome-deficient cells due to its inherent cytotoxicity and interference with the protective action of C18:1.― 267 ―
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