おわりに本研究では，精子膜タンパク質に着目することで受精の必須ステップである透明帯通過の分子機構の一端を解明することができた．今後も，ゲノム編集動物を用いて生殖関連タンパク質の同定と小分子化合物を組み合わせることで，ヒトを含む様々な動物種を対象にした生殖能力の可逆的コントロールを目指して研究開発していきたい．謝 辞本研究遂行に際して，貴財団特別研究助成金 SRG2022 のご支援を賜り，大変有意義な研究活動を実施できたことに深謝いたします．また，弊部研究室員および共同研究者，そしてご指導いただいた先生方に改めて御礼申し上げます．引用文献1. Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, Okabe M., Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa, Proc Natl Acad Sci U S A., 110:8111-8116 (2013).2. Fujihara Y, Okabe M, Ikawa M., GPI-anchored protein complex, LY6K/TEX101, is required for sperm migration into the oviduct and male fertility in mice, Biol Reprod., 90:60 (2014).3. Fujihara Y, Noda T, Kobayashi K, Oji A, Kobayashi S, Matsumura T, Larasati T, Oura S, Kojima-Kita K, Yu Z, Matzuk MM, Ikawa M., Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice, Proc Natl Acad Sci U S A., 116:18498-18506 (2019).4. Fujihara Y, Herberg S, Blaha A, Panser K, Kobayashi K, Larasati T, Novatchkova M, Theussl HC, Olszanska O, Ikawa M, Pauli A., The conserved fertility factor SPACA4/Bouncer has divergent modes of action in vertebrate fertilization, Proc Natl Acad Sci U S A., 118:e2108777118 (2021).5. Fujihara Y (corresponding author), Kobayashi K, Abbasi F, Endo T, Yu Z, Ikawa M, Matzuk MM., PDCL2 is essential for sperm acrosome formation and male fertility in mice, Andrology, 11:789-798 (2023).6. Ye Q, Belabed H, Wang Y, Yu Z, Palaniappan M, Li JY, Kalovidouris SA, MacKenzie KR, Teng M, Young DW, Fujihara Y# (co-corresponding author), Matzuk MM.#, Advancing ASMS with LC-MS/MS for the discovery of novel PDCL2 ligands from DNA-encoded chemical library selections, Andrology, 11:808-815 (2023).7. Herberg S, Gert KR, Schleiffer A, Pauli A., The Ly6/uPAR protein Bouncer is necessary and sufficient for species-specific fertilization, Science, 361:1029-1033 (2018).8. Fujihara Y (corresponding author), Miyata H, Abbasi F, Larasati T, Nozawa K, Yu Z, Ikawa M, Matzuk MM., Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida, PNAS Nexus, 3:108 (2024).― 206 ―
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