AbstractNanocarbon materials have emerged as promising platforms for nucleic acid delivery, as well as bioimaging, and biosensing. Although fullerene derivatives have previously been employed as delivery carriers, their molecular structures have not been precisely optimized for efficient delivery. Here, we report the development of a rationally designed cationic perylene derivative, SMZ052, for effective nucleic acid delivery. SMZ052 exhibits delivery efficiencies comparable to those of widely used lipid-based vectors for both plasmid DNA and siRNA. Mechanistic studies revealed that cellular uptake occurs via both endocytosis and macropinocytosis pathways. Furthermore, SMZ052 enabled successful transfection of plasmid DNA into tobacco BY-2 protoplasts, demonstrating its applicability across biological systems. Our findings highlight the potential of structurally tailored nanocarbon materials as versatile and effective carriers for nucleic acid delivery, offering new avenues for applications in plant and animal biotechnology.はじめに核酸医薬やゲノム編集技術といった核酸を用いた技術は，医学をはじめとする生命科学のあらゆる分野へ革命をもたらした．生命現象の理解と制御のさらなる高度化が必要な今，効率や選択性はもちろんのこと，生体直交性や緻密な時空間制御などの多様な機能が核酸輸送に求められている．核酸輸送の分野に全く新しい方法，キャリア分子群の登場がまたれる所以である．本研究では，我々がこれまで進めてきた「分子ナノカーボン科学」を生命科学分野に利活用する取り組みの一環として，核酸輸送ナノカーボン分子の創製を目指した．理化学研究所開拓研究所・主任研究員Pioneering Research Institute, RIKEN伊丹 健一郎Kenichiro Itami― 13 ―核酸輸送ナノカーボン分子の創製Development…of…molecular…nanocarbonstransporting…nucleic…acids
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