AbstractIn vertebrate development, a single fertilized egg undergoes cell proliferation and differentiation processes to form diverse organs and become an individual. Maternal factors, such as proteins and mRNAs, are replaced by embryonic factors, and metabolic systems such as sugars, amino acids, and lipids change dramatically during the period immediately after fertilization to the mulberry embryo, when cell division is active and cell fate is gradually determined. Although development can proceed in culture medium up to the blastocyst before implantation, the probability that a fertilized egg progresses to a blastocyst in culture medium is only about 60% in IVF, one of the fertility treatments, and the culture method, including the composition of the culture medium, has not been optimized. The analysis of in vitro cultured embryos has shown that normal metabolic conversion is essential for normal embryogenesis, but the dynamics and interactions of the organelle, which is the actual site of metabolism, and the differentiation process are not well understood. However, the dynamics and interactions of organelles, which are the actual sites of metabolism, and their mechanisms of action in determining cell fate during differentiation remain largely unexplored. In this project, we analyzed several organelles such as mitochondria and peroxisomes in early mouse embryogenesis with the aim of understanding the interactions of organelle networks, their regulatory mechanisms and their roles in cell fate determination in a spatio-temporal manner. The imaging analyses showed dramatic changes in dynamics, including clustering, degradation, and proliferation or peroxisomes within four days after fertilization. The clusters were formed by multiple types of organelles. Co-staining experiments with cell fate markers suggest that organelle dynamics is coupled with signal transduction during development.順天堂大学大学院医学研究科難治性疾患診断・治療学・講師Juntendo University杉浦 歩Ayumu Sugiura― 106 ―オルガネラ連関による細胞運命決定機構の解明と生殖医療応用への基盤構築Elucidation of the cell fate determination mechanism through organelle interactions and construction of a foundation for reproductive medicine applications
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